{"id":5005373,"date":"2006-03-15T21:21:55","date_gmt":"2006-03-15T20:21:55","guid":{"rendered":"https:\/\/criticalbiomass.blog.hu\/2006\/03\/15\/dns_origami"},"modified":"2006-03-15T21:21:55","modified_gmt":"2006-03-15T20:21:55","slug":"dns_origami","status":"publish","type":"post","link":"https:\/\/criticalbiomass.hu\/?p=5005373","title":{"rendered":"DNS-origami"},"content":{"rendered":"<p><img src=\"http:\/\/www.nature.com\/nature\/journal\/v440\/n7082\/images\/cover_nature.jpg\" align=\"left\" hspace=\"5\" vspace=\"3\"><br \/>\n\u00c9s akkor <a href=\"http:\/\/criticalbiomass.blog.hu\/2005\/11\/29\/biolego\">BioLego<\/a> ut\u00e1n egy kis DNS-origami.<br \/>\nAz elm\u00falt \u00e9vtizedek eredm\u00e9nyei alapj\u00e1n m\u00e1r sokat tudunk a DNS g\u00f6nd\u00f6r\u00f6d\u00e9s\u00e9r\u0151l, bodorod\u00e1s\u00e1r\u00f3l s egy\u00e9b fizikai tulajdons\u00e1gair\u00f3l, \u00e9s az \u00faj technol\u00f3gi\u00e1knak k\u00f6sz\u00f6nhet\u0151en m\u00e1r kor\u00e1nt sem \u00f6rd\u00f6ng\u00f6ss\u00e9g hosszabb-r\u00f6videbb, tetsz\u0151leges szekvenci\u00e1j\u00fa DNS sz\u00e1lakat szintetiz\u00e1lni. A CalTechen dolgoz\u00f3 <a href=\"http:\/\/www.usc.edu\/dept\/molecular-science\/Paul.htm\">Paul Rothemund<\/a> ezeket felhaszn\u00e1lva j\u00f3pofa k\u00e9t dimenzi\u00f3s \u00e1br\u00e1kat hozott l\u00e9tre DNS-b\u0151l.<br \/>\nKiindul\u00e1sk\u00e9nt az M13 bakteriof\u00e1g (bakteri\u00e1lis v\u00edrus) j\u00f3l ismert egysz\u00e1l\u00fa DNS-\u00e9t haszn\u00e1lta, amit a tervez\u00e9s id\u0151szak\u00e1ban el\u0151bb tetsz\u00e9s szerinti alakzatokba hajtogatott \u00f6ssze. Ezt k\u00f6vet\u0151en, megtervezte a r\u00f6vid komplementer szakaszokat, m\u00e9gpedig \u00fagy, hogy azok gyakran az M13 szekvenci\u00e1b\u00f3l hajtogatott szerkezet szomsz\u00e9dos \u00edveihez csatlakozni k\u00e9pes szekvenci\u00e1b\u00f3l \u00e1lltak, \u00edgy extra stabilit\u00e1st biztos\u00edtva a szerkezetnek. Mint a mell\u00e9kelt \u00e1bra is j\u00f3l mutatja a l\u00e9trehozhat\u00f3 form\u00e1knak szinte&nbsp;csak a k\u00e9pzelet (meg a DNS hossza) szab hat\u00e1rt.<br \/>\n<center><br \/>\n<img src=\"http:\/\/www.nature.com\/nature\/journal\/v440\/n7082\/images\/nature04586-f2.2.jpg\" vspace=\"4\" width=\"600\"><br \/>\n<\/center><br \/>\n\u00c9s ami az extra hab a tort\u00e1n: a fenti technik\u00e1t k\u00fcl\u00f6nb\u00f6z\u0151 DNS-jel\u00f6l\u00e9si elj\u00e1r\u00e1sokkal lehet t\u00e1rs\u00edtani. \u00cdgy egyr\u00e9szt tetsz\u0151leges form\u00e1kba &#8220;bele\u00edrhatunk&#8221; (a nem megjel\u00f6lt b\u00e1zip\u00e1rok digit\u00e1lis &#8220;0&#8221;-k\u00e9nt, m\u00edg a jel\u00f6ltek digit\u00e1lis &#8220;1&#8221;-k\u00e9nt haszn\u00e1lhat\u00f3k), de a DNS b\u00e1zisokat speci\u00e1lis &#8220;kapcsokkal&#8221; is fel lehet szerelni, amelyek az eml\u00edtett 2D-s strukt\u00far\u00e1k szab\u00e1lyos kapcsol\u00f3d\u00e1s\u00e1t teszik lehet\u0151v\u00e9 (l\u00e1sd az al\u00e1bbi k\u00e9pen a h\u00e1romsz\u00f6geket). \u00c9s innen kezdve a hat\u00e1r t\u00e9nyleg a csillagos \u00e9g: tetsz\u0151leges 3D-s szerkezetek is k\u00f6nnyen el\u00e9rhet\u0151k.<br \/>\nH\u00e1t igen, ez v\u00e9gre val\u00f3ban eml\u00e9keztet arra, amit &#8220;nanotechnol\u00f3gia&#8221; n\u00e9ven hirdetnek m\u00e1r \u00e9vek \u00f3ta&#8230;<br \/>\n<center><br \/>\n<img src=\"http:\/\/www.nature.com\/nature\/journal\/v440\/n7082\/images\/nature04586-f3.2.jpg\" vspace=\"4\" width=\"600\"><br \/>\n<\/center><\/p>\n<hr>\n<p><font size=\"1\"><br \/>\n<b>Rothemund, PWK<\/b> (2006) Folding DNA to create nanoscale shapes and patterns. <i>Nature<\/i> <b>440<\/b>, 297\u2013302.<br \/>\n<\/font><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00c9s akkor BioLego ut\u00e1n egy kis DNS-origami. Az elm\u00falt \u00e9vtizedek eredm\u00e9nyei alapj\u00e1n m\u00e1r sokat tudunk a DNS g\u00f6nd\u00f6r\u00f6d\u00e9s\u00e9r\u0151l, bodorod\u00e1s\u00e1r\u00f3l s egy\u00e9b fizikai tulajdons\u00e1gair\u00f3l, \u00e9s az \u00faj technol\u00f3gi\u00e1knak k\u00f6sz\u00f6nhet\u0151en m\u00e1r kor\u00e1nt sem \u00f6rd\u00f6ng\u00f6ss\u00e9g hosszabb-r\u00f6videbb, tetsz\u0151leges szekvenci\u00e1j\u00fa DNS sz\u00e1lakat szintetiz\u00e1lni. A CalTechen &hellip; <a href=\"https:\/\/criticalbiomass.hu\/?p=5005373\">Egy kattint\u00e1s ide a folytat\u00e1shoz&#8230;. <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=\/wp\/v2\/posts\/5005373"}],"collection":[{"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5005373"}],"version-history":[{"count":0,"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=\/wp\/v2\/posts\/5005373\/revisions"}],"wp:attachment":[{"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5005373"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5005373"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/criticalbiomass.hu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5005373"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}